Fig 1: NRBF2 interacted with Rab7 and the MIT domain was indispensable for the interaction between NRBF2 and Rab7. A The lysates from HT22 cells were immunoprecipitated with anti-NRBF2. Then, immunoprecipitates were analyzed by western blotting with anti-NRBF2 and anti-Rab7. n = 5. B Immunostaining for NRBF2 and Rab7 in HT22 cells. n = 3. C Schematic diagram of NRBF2. D Cell lysates were immunoprecipitated with an anti-Rab7 antibody and subjected to western blotting with the indicated antibodies, including anti-NRBF2, -CCZ1, -MON1A, and -Rab7. n = 5